Table 1.

Sequence of primers used for qRT-PCR or MDM2 cloning

Gene primers Sequence	(5′–3′)
qRT-PCR primers
GAPDH F (human)	TGTTCGTCATGGGTGTGAAC
GAPDH R	ATGGCATGGACTGTGGTCAT
E-cadherin F	CGAGAGCTACACGTTCACGG
E-cadherin R	GGGTGTCGAGGGAAAAATAGG
β-catenin F	CATCTACACAGTTTGATGCTGCT
β-catenin R	GCAGTTTTGTCAGTTCAGGGA
N-cadherin F	AGCCAACCTTAACTGAGGAGT
N-cadherin R	GGCAAGTTGATTGGAGGGATG
MDM2 F	GAATCATCGGACTCAGGTACATC
MDM2 R	TCTGTCTCACTAATTGCTCTCCT
Snail F	ACTGCAACAAGGAATACCTCAG
Snail R	GCACTGGTACTTCTTGACATCTG
Slug F	TGTGACAAGGAATATGTGAGCC
Slug R	TGAGCCCTCAGATTTGACCTG
β-actin F (mouse)	CATTGCTGACAGGATGCAGA
β-actin R	CTGCTGGAAGGTGGACAGTGA
E-cadherin F	CTCCAGTCATAGGGAGCTGTC
E-cadherin R	TCTTCTGAGACCTGGGTACAC
β-catenin F	CCCAGTCCTTCACGCAAGAG
β-catenin R	CATCTAGCGTCTCAGGGAACA
N-cadherin F	AGGCTTCTGGTGAAATTGCAT
N-cadherin R	GTCCACCTTGAAATCTGCTGG
MDM2 F	GGATCTTGACGATGGCGTAAG
MDM2 R	AGGCTGTAATCTTCCGAGTCC
Snail F	CTGGGACTCTCTCCTGGTAC
Snail R	GTTGAAGATCTTCCGCGACT
Slug F	TGGTCAAGAAACATTTCAACGCC
Slug R	GGTGAGGATCTCTGGTTTTGGTA
PCR primers for MDM2 clone
MDM2 F	GGGGTACCATGATCTACAGGAACTTGGTAGTAGT
MDM2 R	CCGCTCGAGCTAGGGGAAATAAGTTAGCACAATC

Notes: GAPDH was used as reference control gene for mRNA detection in human tissues and cultured cell lines. β-actin was used as reference control gene for mRNA detection in mice tumor tissues.

Abbreviations: F, forward primer; MDM2, mouse double minute 2; qRT, quantitative reverse transcription; R, reverse primer.