Figure 4.

TRIM31 promoted glioma cell proliferation and invasion through activating NF-κB pathway.

Notes: (A) Luciferase reporter assay showed that TRIM31 overexpression increased NF-κB activity, whereas TRIM31 knockdown inhibited NF-κB activity. (B) Western blot assay showed TRIM31 overexpression increased the nuclear translocation of p65 and the phosphorylation of IkBα, whereas TRIM31 knockdown inhibited these phenotypes; p84 and α-Tubulin were used as the loading control for nuclear proteins and total proteins, respectively. (C) Heat map showed the expression of NF-κB targets after TRIM31 overexpression or knockdown. (D) Colony formation assay showed that inhibition of NF-κB activity in TRIM31 overexpression glioma cells significantly inhibited cell proliferation. (E) BrdU incorporation assay showed that inhibition of NF-κB activity in TRIM31 overexpression glioma cells significantly inhibited cell proliferation. (F) Transwell invasion assay suggested inhibition of NF-κB activity in TRIM31 overexpression glioma cells significantly inhibited cell invasion. Data are reported as mean ± SD. ^*P<0.05.