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. Author manuscript; available in PMC: 2019 Sep 17.
Published in final edited form as: Chem Soc Rev. 2018 Sep 17;47(18):7027–7044. doi: 10.1039/c8cs00278a

Fig. 2.

Fig. 2

Different variations on traditional FRET where: (a) the acceptor releases the obtained energy non-radiatively (dark quenching), (b) the FRET pair consists of identical fluorophores (homo-FRET), (c) the fluorescence lifetime of the donor fluorophore is used to monitor energy transfer (FRET-FLIM), or (d) the donor fluorophore is replaced by a bioluminescent protein (red) to thereby eliminate the need for external illumination (BRET). The depicted excitation and emission wavelengths serve as an example.