Value of the data •This particular Bacillus sp. strain VKPM B-3276 was isolated from Culex pipiens larvae and showed significant entomopathogenic activity [2], therefore could be regarded as prospective entomocide. •The genome encodes a high number of various enzymes, participating in chitin and peptidoglycan degradation, which could be relevant in medicine (antimicrobial agents) or for waste utilization (chitin bioconversion). •According to whole genome alignment data Bacillus sp. strain VKPM B-3276 may be regarded as a new subspecies inside “Bacillus cereus group”. •Data on genome sequence of strain VKPM B-3276 can be used to search and characterize novel biotechnology-relevant enzymes and gene clusters.