Table 1. Primary antibodies and protocols for immunohistochemical analysis.
| Antibody | Clone | Isotype | Manufacturer | Antigen retrieval | Dilution | Incubation |
|---|---|---|---|---|---|---|
| ER-α | 1D5 | Mouse IgG1 | DAKO | Citric acid, 20 min | 1:100 | 4°C, overnight |
| ER-β | PPG5/10 | Mouse IgG2a | Bio-Rad | Citric acid, 10 min | 1:80 | 4°C, overnight |
| PR | PR10A9 | Mouse IgG2a | Immunotech | Tris-EDTA, 15 min | 1:500 | 4°C, overnight |
| CD3 | Polyclonal | Rabbit polyclonal | DAKO | Tris-EDTA, 5 min | 1:250 | RT, 1h 30min |
| CD79a | HM57 | Mouse IgG1k | DAKO | Citric acid, 10 min | 1:600 | RT, 1h 30min |
| IL-1α | B-7 | Mouse IgG2b | Santa Cruz Biotechnology | Citric acid, 15 min | 1:600 | RT, 1h 30min |
| IL-1β | H-153 | Rabbit polyclonal IgG | Santa Cruz Biotechnology | Citric acid, 20 min | 1:200 | RT, 2h |
| IL-6 | M-19 | Goat polyclonal IgG | Santa Cruz Biotechnology | Tris-EDTA, 15 min | 1:400 | RT, 1h 30min |
| IL-8 | 807 | Mouse IgG1 | Abcam | Tris-EDTA, 5 min | 1:250 | RT, 1h 30min |
ER, estrogen receptor; Ig, immunoglobulin; PR, progesterone receptor; CD, cluster of differentiation; IL, interleukin; RT, room temperature.