Fig. 4.

The V-ATPase GBM-like signature associates with de-differentiation and upregulation of homeobox genes. a, b) DAVID analysis of pathways associated with LGG/IDHmut (a) or GBM-like (b) classes in LGG/IDHwt cases. The complete list of genes used to generate these plots is provided in Tables S15, S16. c) Heatmap showing epigenetic enzymes expression in LGG/IDHwt cases grouped according to the V-ATPase class. For each gene the p value (Kolmogorov-Smirnov test) is indicated. The distribution of homeobox genes expression in each sample is provided at the bottom. d) Selected homeobox genes were analyzed in an independent set of LGG/IDHwt patients grouped according to the V-ATPase class (Gravendeel series; n = 40). **, p = 0·001; *, p = 0·02; #, p = 0·03; §, p = 0·006; °, p = 0·04 (Mann-Whitney U test). Data are presented as notched box plots (lower, median and upper quartile) with whiskers indicating the minimum and maximum values. Each circle represents a sample.