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. 2019 Feb 5;41:214–224. doi: 10.1016/j.ebiom.2019.01.052

Fig. 5.

Fig. 5

NS with elevated V1G1 levels are characterized by upregulation of Homeodomain-containing genes. a) The indicated transcripts were analyzed by qPCR in primary NS or differentiated cultures. Bars, mean ± SEM (n = 4). b,) Representative NS cultures with high (NS92 and 97) or low (NS88 and 121) V1G1 expression were analyzed for the indicated proteins expression by western blotting. Vinculin was a loading control. c) The homeodomain proteins HOXA10, HOXA7 or POU3F2 were analyzed by immunofluorescence in NS with Low or High V1G1 levels. Scale bars, 50 μm.