Figure 3.
TARBP2 protein stability is decreased in HCC/SR cells. (A, B) The expression of TARBP2 mRNA was determined via real‐time PCR in paired Huh7 (A) or PLC5 (B) cell lines. Multigroup comparisons were analyzed by one‐way ANOVA with Tukey's post hoc test. (C–F) The TARBP2 protein was destabilized in HCC/SR cells. Huh7 and Huh7/SR (C) or PLC5 and PLC5/SR (E) cells were treated with CHX (100 μg·mL−1) for the indicated time periods. The relative quantity of the depicted proteins was analyzed through three independent experiments (D, F). Data are presented as mean ± SEM, with at least n = 3 per group. Multigroup comparisons were analyzed by two‐way ANOVA with Tukey's post hoc test. P values < 0.05 were considered statistically significant. ***P < 0.005.