Table 1.
Cardiac parameters obtained by echocardiography at baseline and after dobutamine treatment of CON and PUN female and male mice at PN80
| Female | Male | P | ||||||
|---|---|---|---|---|---|---|---|---|
| CON | PUN | CON | PUN | |||||
| n | 9 | 8 | 6 (3)2 | 6 (3)2 | Diet × Sex1 | Diet | Sex | |
| LV mass (mg) | 67.9 ± 5.2 | 51.9 ± 5.5 | 101.9 ± 6.3 | 84.6 ± 6.3 | n.s. | 0.01 | <0.001 | |
| LV mass (mg/g lean) | 3.80 ± 0.29 | 3.37 ± 0.31 | 4.45 ± 0.36 | 4.16 ± 0.36 | n.s. | n.s. | 0.04 | |
| LV posterior wall (mm) | 0.66 ± 0.05a | 0.48± 0.05b | 0.87 ± 0.06c | 0.87 ± 0.06c | 0.1 | |||
| LV anterior wall (mm) | 0.71 ± 0.05a | 0.53± 0.05b | 0.64 ± 0.05a | 0.68 ± 0.05a | 0.04 | |||
| LV diastole vol (μL) | B | 67.6 ± 2.8 | 59.1 ± 3.0 | 84.3 ± 3.5 | 68.5 ± 3.5 | n.s. | 0.001 | <0.001 |
| +Db | 59.1 ± 2.4 | 50.2 ± 2.5 | 59.4 ± 4.1 | 51.8 ± 4.1 | n.s. | 0.04 | n.s. | |
| Stroke volume (μL) | B | 42.7 ± 1.7 | 36.9 ± 1.8 | 47.7 ± 2.1 | 45.4 ± 2.1 | n.s. | 0.05 | 0.002 |
| +Db | 43.6 ± 1.8 | 37.8 ± 1.9 | 45.1 ± 3.1 | 42.8 ± 3.1 | n.s. | 0.04 | n.s. | |
| FS (%) | B | 37.6± 1.7b | 31.6 ± 1.7ab | 29.5 ± 2.0a | 37.4 ± 2.0b | 0.001 | ||
| +Db | 44.4 ± 2.4 | 42.4 ± 2.6 | 44.1 ± 4.2 | 53.0 ± 4.2 | n.s. | n.s. | n.s. | |
| Cardiac index3 (cm/s) | B | 27.6 ± 1.2 | 25.7 ± 1.2 | 30.4 ± 1.4 | 31.4 ± 1.3 | n.s. | n.s. | 0.01 |
| +Db | 39.2 ± 1.5a | 33.7 ± 1.5b | 49.6 ± 2.4c | 47.9 ± 2.4c | 0.1 | |||
Values are means ± SEM for mice at baseline (B) and when stimulated with 1.5 μg dobutamine/g BW (+Db).
1Where P ≤ 0.1 for diet × sex interaction, post hoc analysis was performed to test for the effect of sex within diet, or diet within sex; significance is indicated by superscripts a, b, and c, such that P < 0.05 when means do not share the same superscript; n.s., not significant.
2Dobutamine injections were successful in only 3 male mice/dietary treatment.
3From mean aortic velocity (by Doppler).