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. 2019 Mar 1;597(7):1919–1934. doi: 10.1113/JP277617

Figure 1. Injection of ACh into the RTN increased breathing and blood pressure in awake unrestrained rats by a muscarinic cholinergic receptor‐dependent mechanism.

Figure 1

A, histology section showing ChAT immunoreactivity at the level of the RTN region. B, cardiovascular and ventilatory responses elicited by ACh injection into the RTN of awake unrestrained rats before and after unilateral RTN injections of saline or M‐Atr. C, histology section showing the location of drug injections within the RTN. D–G, summary data (n = 5 rats) showing the effect of unilateral RTN injections of saline (Sal; 50–75 nL), ACh (10 mm/50 nL) and ACh plus M‐Atr (5 mm/50–75 nL) on V T (D); f R (E); V E (F); and MAP (G) in awake unrestrained rats. VII, facial motor nucleus; VMS, ventral medullary surface. Scale bar = 100 μm in (A) and (C) and 50 μm in (A'). *Statistically different from both resting and M‐Atr (one‐way RM ANOVA with Bonferroni´s correction for multiple comparisons). [Color figure can be viewed at wileyonlinelibrary.com]