Table 1.

Single cell RT‐PCR primer sequences

Gene	Primer	Sequence	Product length
β Actin	Forward (5′‐3′) Reverse (3′‐5′)	CTG GTC GTC GAC AAC GGC TCC GCC AGA TCT TCT CCA TG	302 bp
S1PR1	Forward (5′‐3′) Reverse (3′‐5′)	TCT GCT CCT GCT TTC CAT CG AGG ATG TCA CAG GTC TTC GC	237 bp
S1PR2	Forward (5′‐3′) Reverse (3′‐5′)	CCT TCG TGG CCA ACA CCT TA TGT CAC TGC CGT AGA GCT TG	177 bp
S1PR3	Forward (5′‐3′) Reverse (3′‐5′)	ACC GCG TGT TCC TTC TGA TT TTG ACC AGG CAG TAG ATG CG	214 bp
S1PR4	Forward (5′‐3′) Reverse (3′‐5′)	GTG TAT GGC TGC ATC GGT CT CCA CTA GGA TGA GGG CGA AG	169 bp
S1PR5	Forward (5′‐3′) Reverse (3′‐5′)	TGT GTG TGC CTT CAT TGT GC ACA GTA GGA TGT TGG TGG CG	152 bp
P2X2	Forward (5′‐3′) Reverse (3′‐5′)	GGG GCA GTG TAG TCA GCA TC TCA GAA GTC CCA TCC TCC A	241 bp
TRPV1	Forward (5′‐3′) Reverse (3′‐5′)	TCA CCG TCA GCT CTG TTG TC GGG TCT TTG AAC TCG CTG TC	285 bp