Skip to main content
. 2019 Jan 30;13(4):840–856. doi: 10.1002/1878-0261.12437

Figure 6.

Figure 6

LINC01436 functions as a sponge for miR‐30a‐3p and exerted its function through miR‐30a‐3p. (A) Genome‐wide miRNA expression in the same five paired lung tumor tissues and adjacent normal lung tissues were investigated by microarray analysis. Hierarchical cluster analysis of significantly differentially expressed miRNA: bright red, up‐regulation; bright green, down‐regulation. (B) Potential miRNA were predicted to target LINC01436 by the miRanda algorithm (upper). A Pearson correlation was performed to analyze the correlations between LINC01436 and three potential target miRNA (lower). (C) LINC01436 expression levels in A549 cells after transfection with miR‐30a‐3p inhibitors or miR‐30a‐3p mimics. (D) MiR‐30a‐3p expression levels were detected in A549 cells after overexpression or knockdown of LINC01436. (E) Luciferase assays of A549 cells co‐transfected with LINC01436‐WT or LINC01436‐MUT reporter and miR‐30a‐3p mimics or negative control (NC) mimics. (F) Migration of A549 cells after co‐transfection with LINC01436 or control vector and miR‐30a‐3p mimics or NC mimics. Magnification, ×200. Error bars represent the SD of three independent experiments. *Student's t‐test, P < 0.05; **Student's t‐test, P < 0.01; #Student's t‐test, P > 0.05.