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. 2019 Mar 28;25(12):1465–1477. doi: 10.3748/wjg.v25.i12.1465

Figure 3.

Figure 3

Histological analysis confirms the strength of the dextran sulfate sodium model. A: Hematoxylin and eosin staining of colon sections of mice from 4 groups of treatment at day 5 and 12. Forty-fold magnification was used. B: Protein expression of tight junctions occludin and ZO-1 in colon tissue. Antibody anti-β-actin was used as endogenous control. On the right, the histograms represent the mean of densitometry values from 3 different experiments. C: CD3 infiltration in colon mucosa was assessed by quantitative PCR of CD3 mRNA. One-way ANOVA (and non-parametric) was calculated; aP < 0.05; bP < 0.01; cP < 0.0001. DSS: Dextran sulfate sodium; TNFα: Tumor necrosis factor α.