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. 2019 Mar 28;25(12):1465–1477. doi: 10.3748/wjg.v25.i12.1465

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©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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T cells subsets characterization in presence of an anti-tumor necrosis factor α agent. T cells were isolated from mesenteric lymphnode of mice and CD4⁺ cells were studied by flow cytometry. In particular, CD4⁺ regulatory T lymphocytes characterization was possible through the use of anti-CD25 and anti–FoxP3 antibodies (B); type 1 helper T lymphocytes expressed interferon (IFN)γ and tumor necrosis factor α (C); IFNγ⁺ and interleukin 17⁺ cells identified the type 17 helper T lymphocytes cluster (D). In each panel (B), (C) and (D) the cells in the upper right quadrant were the double staining cells. Treg: CD4⁺ regulatory T lymphocytes; Th1: Type 1 helper T lymphocytes; Th17: Type 17 helper T lymphocytes; DSS: Dextran sulfate sodium; TNFα: Tumor necrosis factor α; IFNγ: Interferon γ; IL: Interleukin.