Table 1.
Sequences of primers and probes
| 5ʹ primer | 3ʹ primer | Taqman probe | Efficacy (%) | |
|---|---|---|---|---|
| TRPV1 | CCGGAGAGATCTTGTCTGTG | ACCAGCATGAACAGCGACT | TATTTCCTGCAGAGGCGACCATCC | 95.7 |
| TRPA1 | ATCAGGAGACCCTGCTTCAC | GTTGATGTCTGCTCCCACTG | TGATCATCATGACCTGGCAGACTACC | 101.3 |
| Kv 1.4 | TGACCTACTGCCACAGGATG | GGTTTCGAAGCGTAGACCAG | CAGTGACTGTTGTGAACGCGTGGT | 104.4 |
| P2X2 | GCATCATCACCAGGATCGAG | GTCTTGGAGTCCCCATGGTA | TCAGACGACGACTGTATTGCCGGA | 102.2 |
| P2X3 | GGCTGGATGGAGTTTCTGAG | TTCAGGAGTGTGCGGTACTC | CCCTGGCTACAACTTCAGGTTTGCC | 104.4 |
| GAPDH | AGACAGCCGCATCTTCTTGT | GATACGGCCAAATCCGTTC | GCAGTGCCAGCCTCGTCTCA | 104.0 |