Skip to main content
. 2019 Feb 7;41:256–267. doi: 10.1016/j.ebiom.2019.01.066

Fig. 3.

Fig. 3

FA 2-hydroxylation regulates Gli1 level and sensitivity to cisplatin.

a, b) MKN45 and SGC7901 cells were untreated (CTL) or transfected with negative control (NC) siRNA or siRNA against FA2H (FA2H KD). The whole cell lysates were prepared and subjected to Western blot analysis with antibodies directed against each specific protein as indicated (a). (b)The bands were quantified and presented as the mean ± SEM (n = 3).

c, d) MKN45 and SGC7901 cells were untreated or transfected with empty vector (Vec) or plasmid encoding hFA2H (FA2H OE). The whole cell lysates were prepared and subjected to Western blot analysis with antibodies directed against each specific protein as indicated (c). (d) The bands were quantified and presented as the mean ± SEM (n = 3).

e, f) MTT assay of SGC7901 and MKN45 cells in response to cisplatin. (e) Cells were transfected with negative control (NC) siRNA or siRNA against FA2H (FA2H KD). (f) Cells were transfected with empty vector (Vec) or plasmid encoding hFA2H (FA2H OE). Results presented as mean ± SEM (n = 3). *, P < 0·05; **, P < 0·01; ***, P < 0·001.