Fig. 5.

FA 2-hydroxylation regulates AMPK phosphorylation.

a, b) SGC7901 and MKN45 cells were untreated (CTL) or transfected with negative control (NC) siRNA or siRNA against FA2H (FA2H KD). (a) The whole cell lysates were prepared and subjected to Western blot analysis with antibodies directed against each specific protein as indicated. (b) The bands were quantified and presented as the mean ± SEM (n = 3).

c, d) SGC7901 and MKN45 cells were untreated (CTL) or transfected with empty vector (Vec) or plasmid encoding hFA2H (FA2H OE). (c) The whole cell lysates were prepared and subjected to Western blot analysis with antibodies directed against each specific protein as indicated. (d) The bands were quantified and presented as the mean ± SEM (n = 3).

e, f) SGC7901 and MKN45 cells were untreated (CTL) or pre-treated with BSA, 50 μM PA, (R)-2-OHPA (2R), or (S)-2-OHPA (2S). (e) The whole cell lysates were prepared and subjected to Western blot analysis with antibodies directed against each specific protein as indicated. (f) The bands were quantified and presented as the mean ± SEM (n = 3).

*, P < 0·05; **, P < 0·01, ns, not significant.