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. 2019 Feb 13;41:584–596. doi: 10.1016/j.ebiom.2019.02.001

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Reconstitution of human immunity in hIL-6 Tg NSG mice.

(a) Representative flow cytometry plots of a hIL-6 Tg NSG humanised mouse (IL6#1–1) demonstrating the engraftment of human CD19⁺ B cells, CD33⁺ myeloid cells, CD3⁺ T cells, and CD56⁺ NK cells in BM and spleen. (b) Representative flow cytometry plots showing splenic Treg subsets in a hIL-6 Tg NSG humanised mouse (IL6#1–1). Foxp3⁺ Tregs are classified into CD45RA⁺Foxp3^lo naïve Tregs (I), CD45RA⁻Foxp3^hi effector Tregs (II), and CD45RA⁻Foxp3^lo non-Tregs (III). (c) Frequency of Foxp3⁺ Tregs in the spleen are lower in hIL-6 Tg NSG humanised mice (NSG: n = 11, IL-6: n = 14), with significant reduction in naïve Tregs and effector Tregs (NSG: n = 11, IL-6: n = 7) (*p = 0.0004, **p = 0.02, ***p = 0.03). Error bars represent mean ± SEM. (d) T cell-dominant engraftment was found in the skin of hIL-6 Tg NSG humanised mouse as shown by representative flow cytometry plot (IL6#1-1).