Fig. 2.

Histological analysis of cGVHD humanised mice.

Histological analysis of (a) skin, (b) lung and (c) liver from hIL-6 Tg NSG humanised mice with a hIL-6 non-Tg NSG humanised mouse as control. (a) H&E and immunohistochemical staining is consistent with interface dermatitis with epidermal thickening associated with infiltration of CD4⁺ and CD8⁺ T cells in cGVHD humanised mice. White arrow shows sclerotic and thickened upper dermis (IL6#2-1). (b) Pulmonary sections of the same recipients show infiltration of CD4⁺ T and CD8⁺ T cells. (c) Infiltration of human CD45⁺ leucocytes including CD3⁺ T cells is detected in the liver of cGVHD humanised mice. In contrast, a non-Tg NSG humanised mouse (NSG#12-1) shows normal skin, lung and liver histology. Scale bars: Low magnification 100 μm; high magnification 50 μm.