Fig. 2.

Gliomas with DDR alterations exhibit distinct infiltrating immune cells phenotypes and cytokines expressions.

(a) CIBERSORT scores were estimated with normalized gene expression data to infer the relative proportions of twenty-two types of immune cells. The proportions of twenty-two immune cells were depicted in a summary of all TCGA samples by stacked bar charts and their distributions were represented in box plots. (b) IHC assays were performed to detect the M2 microglia markers CD68, CD163, CD204 and CD206 in clinical glioma tissues (20×). (c) Overlap of cytokines associated with poor outcomes in the TCGA and CGGA datasets. (d-e) Cox regression analyses of DDR-related cytokines in GME for the patients with GBM in the TCGA and CGGA datasets.