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. 2019 Apr 1;22(3):e25268. doi: 10.1002/jia2.25268

Figure 3. HIV‐1 replication enhances HCV transmission by LCs ex vivo.

Figure 3

(A‐B) Epidermal sheets were floated on medium in a 24‐well plate and exposed to either HIV‐1 (JRCSF) or HIV‐1 (SF162) for 24 hours and subsequently exposed to infectious HCV (JFH1‐AM120‐Rluc) for another 24 hours. Emigrated cells were harvested, extensively washed, co‐cultured with huh7.5 cells and analysed for luciferase reporter activity. (A) Each dot represents 1 donor. Horizontal bars are the means of n=10 donors measured in triplo or quadruplo. ***p < 0.001 by two‐tailed, paired Student's t‐test. (B) Epidermal sheets were pre‐exposed to HIV‐1 replication inhibitors Raltegravir or Indinavir for two hours. Each dot represents 1 donor. Horizontal bars are the means of n=7 donors measured in quadruplo. *p < 0.05, **p < 0.01, by two‐tailed, paired Student's t‐test. (C) Epidermal sheets were pre‐exposed to HIV‐1 replication inhibitors Raltegravir or Indinavir for two hours, exposed to either medium only or HIV‐1 (JRCSF) for 48 hours. Emigrated LCs were harvested, stained with an antibody against CD1a and absolute cell count beads and analysed by flow cytometry. Error bars are the mean ± SD of n=2 donors measured in duplo. ns, not significant, by two‐tailed, unpaired Student's t‐test. HCV, Hepatitis C virus; IDV, Indinavir; RAL, Raltegravir; UI, uninfected.