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. 2019 Apr 1;22(3):e25268. doi: 10.1002/jia2.25268

Figure 4. Mature and HIV‐1‐infected LCs retain infectious HCV for transmission.

Figure 4

(A) Epidermal sheets were exposed to HIV‐1 (JRCSF) for 24 hours and subsequently exposed to pseudotyped HCV (HIV‐1 NL4.3Δenv pseudotyped with HCV env glycoproteins E1 and E2) for another 24 hours, extensively washed, co‐cultured with huh7.5 cells and analysed for luciferase reporter activity. Horizontal bars are the means of n=7 donors measured triplo and quadruplo. **p < 0.01, by two‐tailed, paired Student's t‐test. (B) Mature LCs were exposed to pseudotyped HCV (HIV‐1 NL4.3Δenv pseudotyped with HCV env glycoproteins E1 and E2) for 24 hours, extensively washed, co‐cultured with huh7.5 cells and analysed for luciferase reporter activity. Horizontal bars are the means of n=14 donors measured in duplo, triplo and quadruplo. ****p < 0.0001, by two‐tailed, paired Student's t‐test. (C) Immature and mature LCs from the same donor were isolated, exposed to pseudotyped HCV (HIV‐1 NL4.3Δenv pseudotyped with HCV env glycoproteins E1 and E2) for 24 hours, extensively washed, co‐cultured with huh7.5 cells and analysed for luciferase reporter activity. Error bars are the mean ± SD of n=3 donors measured in triplicates. ***p < 0.001, by two‐tailed, paired Student's t‐test. HCV, Hepatitis C virus; iLC, immature Langerhans cell; mLC, mature Langerhans cell; UI, uninfected.