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. 2019 Feb 5;294(13):5214–5227. doi: 10.1074/jbc.RA118.005828

Table 1.

Kinetic affinity constants of the mTNF, hTNF, mLTα, and hLTα binding by CrmD mutants

CrmD genotype variants are shown in the left column. The association constant (Ka), dissociation constant (Kd), and their respective standard errors (S.E.) for each cytokine are indicated. The binding affinity constant (KD, in bold) is shown in nm units. nbd, no binding detected.

CrmD mTNF
 hTNF
 mLTα
 hLTα
Ka ± S.E.a Kd ± S.E.b KD Ka ± S.E. Kd ± S.E. KD Ka ± S.E. Kd ± S.E. KD Ka ± S.E. Kd ± S.E. KD
WT 3.27 ± 0.02 0.33 ± 0.07 0.10 9.64 ± 0.03 3.94 ± 0.09 0.41 2.31 ± 0.01 9.86 ± 0.11 4.27 0.60 ± 0.01 30.10 ± 0.27 50.40
D35A/Y36A 3.75 ± 0.02 6.50 ± 0.70 1.73 20.80 ± 0.31 45.00 ± 2.20 2.16 2.72 ± 0.04 43.30 ± 1.09 15.90 nbd nbd nbd
K44A 2.81 ± 0.01 0.29 ± 0.07 0.10 8.49 ± 0.03 3.24 ± 0.11 0.40 1.20 ± 0.02 5.93 ± 0.22 4.95 0.40 ± 0.01 30.40 ± 0.26 75.80
P68A 4.07 ± 0.01 0.61 ± 0.06 0.15 11.10 ± 0.03 2.90 ± 0.08 0.26 2.66 ± 0.03 12.60 ± 0.34 4.71 1.22 ± 0.01 23.60 ± 0.24 19.30
F72A/T73A/S74A nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd
N77A 4.75 ± 0.02 2.59 ± 0.11 0.55 15.60 ± 0.26 45.90 ± 1.33 2.47 0.22 ± 0.03 13.90 ± 0.61 62.00 5.56 ± 0.20 411.00 ± 8.39 73.90
N77F nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd
H78A/S79A 2.97 ± 0.03 2.39 ± 0.33 0.80 16.10 ± 0.06 8.19 ± 0.16 0.51 3.00 ± 0.04 6.12 ± 0.10 2.04 4.81 ± 0.07 55.80 ± 0.68 11.60
R86A/K88A 3.13 ± 0.02 2.30 ± 0.45 0.73 11.60 ± 0.04 6.28 ± 0.10 0.54 0.84 ± 0.01 6.19 ± 0.21 7.02 6.09 ± 0.11 82.50 ± 0.82 13.50
S90A/S91A 2.34 ± 0.01 2.36 ± 0.06 1.00 15.90 ± 0.08 11.10 ± 0.16 0.70 1.25 ± 0.01 8.58 ± 0.14 6.99 0.49 ± 0.01 20.10 ± 0.31 41.10
Q93A/E95A 3.19 ± 0.01 2.87 ± 0.11 0.90 15.40 ± 0.10 17.00 ± 0.22 1.11 2.84 ± 0.03 32.00 ± 0.64 11.20 nbd nbd nbd
Q103A/D104A/R105A nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd nbd
E116A/F117A/E118A 2.29 ± 0.01 1.93 ± 0.64 0.84 10.70 ± 0.09 12.50 ± 1.35 1.17 2.38 ± 0.03 13.30 ± 0.22 5.58 5.08 ± 0.02 11.30 ± 0.09 2.22
Y141A/S142A/S143A 3.05 ± 0.03 2.62 ± 0.10 0.86 14.70 ± 0.12 32.70 ± 0.96 2.23 0.41 ± 0.01 20.70 ± 0.28 50.60 0.30 ± 0.01 50.70 ± 0.99 107.00
D146A/I148A 4.91 ± 0.03 4.35 ± 0.45 0.89 21.20 ± 0.22 31.00 ± 1.28 1.46 3.22 ± 0.05 46.00 ± 1.51 14.30 1.15 ± 0.04 312.00 ± 8.15 271.00

aKa ± S.E. × 105 (1/m s).

b Kd ± S.E. × 10−4 (1/s).