Figure 2.

Contribution of CopZ1 and CopZ2 to P. aeruginosa Cu⁺ tolerance and metal content. A, growth rate of WT, ΔcopZ1, ΔcopZ2, ΔcopZ1/ΔcopZ2, ΔcopA1, as well as copZ1 and copZ2 complemented strains in the presence of 0–4 mm CuSO₄. B, intracellular Cu levels before (ochre) and after (black) 10 min exposure to 0.5 mm CuSO₄. Data are the mean ± S.E. of three independent experiments. Significant differences from values in the WT strain as determined by unpaired two-tailed Student's t test are: **, p < 0.01, ***, p < 0.001.