Figure 3.

Cu⁺ exchange among CopZ1 and CopZ2. A and B, protein (black) and Cu⁺ (green) in the washes and eluates from Ni-NTA resin (wash 1–4, elution 5–7). A, Cu⁺ transfer from CopZ1_holo in the absence (left) and presence of CopZ2_apo (right); and B, Cu⁺ transfer from CopZ2_holo in the absence (left) and presence of CopZ1_apo (right). Data are the mean ± S.E. of three independent experiments. C, Cu⁺ transfer reaction from CopZ1_holo to CopZ2_apo after various incubation times expressed as a reaction quotient Q = ([CopZ1_apo] × [CopZ2_holo] × [CopZ1_holo]⁻¹ × [CopZ2_apo]⁻¹) for CopZ1_holo + CopZ2_apo ⇋ CopZ1_apo + CopZ2_holo.