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Cu+ transfer from CopZsholo to CueRapo. Experiments were performed in the absence (A) and presence (B) of PcopZ2. Data are from a representative Cu+ transfer experiment from CopZ1holo (left) and CopZ2holo (right). Protein (black) and Cu+ (green) contents are shown in washes and eluates from Ni-NTA resin (wash 1–6, elution 7–9). Lower panels show fractions analyzed for DNA content (top panel), Strep-tagged CueR (second panel), and His-tagged CopZs (bottom panel).
