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. 2019 Feb 4;294(13):4934–4945. doi: 10.1074/jbc.RA118.006316

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© 2019 Novoa-Aponte et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — In vitro interaction between CopZs and CueR. Co-purification of isolated proteins by Strep-Tactin®XT batch affinity chromatography using 10 μm Strep-tagged CueR_apo dimer and varying concentrations of His-tagged CopZs_apo (10–100 μm). His-tagged proteins co-purified with CueR were immunostained to calculate their relative abundance as a measure of the fraction of CueR bound to CopZs (CopZ1, ochre, and CopZ2, black). Curves were fitted to a sigmoidal equation giving a K_½ value of 1:4.73 ± 0.13 for CopZ1 and ≥1:9 for CopZ2 (units are CueR:CopZ ratios). Data are the mean of two replicates of an immunostaining representative from three independent experiments.