Figure 8.

In vivo abundance of CopZ1 (ochre) and CopZ2 (black) proteins. ΔcopZ1 and ΔcopZ2 were complemented with the corresponding His-tagged gene under control of the native promoter. His-tagged proteins were immunostained and their abundance calculated using a standard curve of pure His-tagged protein. The horizontal dividing lines indicate where the images have been spliced; signals were from an identical original image and have not been altered. CopZs levels were determined after exposure to (A) different CuSO₄ concentrations during 10 min or (B) 0.5 mm CuSO₄ during different times. Data are the mean ± S.E. of three independent experiments. Significant differences from values in the absence of CuSO₄ as determined by unpaired two-tailed Student's t test were: **, p < 0.01.