Figure 4.
A, immunoblot analysis of solubilized cell envelope fractions of WT M. marinum and a mycP5 knockout, complemented with WT mycP5 (P5), hybrid constructs of mycP5 with the TM region of mycP1 (P5TM1) or mycP1 with the TM region of mycP5 (P1TM5), WT mycPP1 (P.P1), and a hybrid construct of mycPP1 with the TM region of mycP5 (P.P1-TM5). Proteins were visualized with anti-EccB5 and, as loading controls, anti-EccB1 and anti-FtsH. B, blue native PAGE immunoblot analysis of solubilized cell envelope fractions; the ESX-5 complex was visualized with anti-EccB5.