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. 2019 Feb 1;294(13):4738–4758. doi: 10.1074/jbc.RA118.005008

Figure 4.

Figure 4.

Phosphodeficient RAB8A, but not WT or phosphomimetic RAB8A variants, revert the LRRK2-mediated effects on EGFR trafficking. A, HeLa cells were transfected with either empty pCMV vector (ctrl) or the indicated RAB8A constructs, and surface-bound fluorescent EGF was quantified. n = 4 independent experiments. *, p < 0.05. B, cells were transfected with the indicated constructs followed by quantification of internalized fluorescent EGF. n = 4 independent experiments. *, p < 0.05. C, cells were cotransfected with G2019S LRRK2 and the indicated RAB8A constructs, and surface-bound fluorescent EGF was quantified. n = 8 independent experiments. *, p < 0.05. D, cells were transfected with the indicated constructs, and internalized fluorescent EGF was quantified at 10 (left) and 30 min (right). n = 8 independent experiments. *, p < 0.05; ***, p < 0.005; ****, p < 0.001. All error bars represent S.E.M.