Figure 10.

miR-144-3p controls fibrotic gene expression in lung fibroblasts. Donor and IPF lung fibroblasts (n = 4) were treated with miR-144-3p mimic or scrambled control and anti-miR-144-3p mimic or negative control inhibitor, respectively. RNA was isolated and processed for qPCR for (A, A′), COL1A2 (B, B′), COL3A1 (C, C′), ACTA2 (D, D′), and VCAN (E, E′) FN1. Transfection of mimic resulted in significantly higher basal levels of COL1A2, COL3A1, ACTA2, VCAN, and FN1 mRNA in donor lung fibroblasts compared with scrambled controls, whereas transfection of antagomir resulted in significantly lower basal levels of COL1A2, COL3A1, ACTA2, VCAN, and FN1 in IPF lung fibroblasts compared with a negative control inhibitor (paired, two-tailed Student's t test, n = 4).