AP-1 complex is one of the key regulators of miR-144 locus. Quantitative RT-PCR for miR-144-3p following silencing of c-Jun and/or c-Fos in (A) donor or (B) IPF lung fibroblasts (data were analyzed by two-way ANOVA, followed by Tukey's post hoc test, n = 4). Experiments with donor and IPF lung fibroblasts were performed in parallel, and data from A and B were analyzed independently. C and D, quantitative RT-PCR for silencing of c-Jun (C) and c-Fos (D) from A and B, respectively. Data were analyzed by two-way ANOVA, followed by Tukey's post-hoc test, n = 4. E and F, quantitative RT-PCR for c-Jun (E) and c-Fos (F) between unstimulated donor and IPF lung fibroblasts. Data were analyzed by unpaired t test, n = 6.