Figure 7.
Binding of CCHFV N protein with other Bunyavirus panhandles. Binding profiles generated by filter-binding analysis for the association of WT CCHFV N protein (A), head domain (C), and stalk domain (E) with the hazara virus S-segment panhandle, composed of 30 nucleotides from both 5′ and 3′ termini of hazara virus S-segment vRNA separated by a six-residue uracil loop. The corresponding binding profiles with a 30-nucleotide long 5′ NCR sequence (ssRNA) of CCHFV S-segment vRNA are shown in B, D, and F, respectively. The binding profiles were generated as described under “Experimental procedures.” The dissociation constants (Kd) calculated from each binding profile are shown in the corresponding panel. Binding profiles for the interaction of WT CCHFV N protein and stalk domain with hantavirus S-segment panhandle are shown in G and H, respectively.