Skip to main content
. 2019 Feb 5;294(13):5023–5037. doi: 10.1074/jbc.RA118.004976

Figure 8.

Figure 8.

Effect of CCHFV N protein expression upon hazara virus replication. HEK293T cells seeded in six-well plates were transfected with empty pTriEx1.1 plasmid, pTriEx-CCHFV-NP plasmid, pTriExhead plasmid, pTriExhead-mutant34 plasmid, pTriExstalk plasmid, pTriExstalk-mutant plasmid, pTriEx CCHFV N protein mut1 plasmid, or pTriEx CCHFV N protein mut2 plasmid, followed by infection with hazara virus 12 h post-transfection at an m.o.i. of 1.0, as described under “Experimental procedures.” Cells were harvested at increasing time points post-infection. Hazara virus replication was monitored by quantitative estimation of S-segment vRNA by real time PCR analysis (A). Western blot analysis was performed to examine the expression of C-terminally His-tagged proteins expressed from transfected plasmids, using anti-His tag antibody (B). HUVEC cells in six-well plates were similarly transfected with empty pTriEx1.1 plasmid, pTriExhead plasmid, or pTriExstalk plasmid, followed by infection with Andes virus at an m.o.i. of 1.0. Andes virus replication was monitored by quantification of S-segment vRNA by real time PCR (C). Expression of stalk and head domains was examined by Western blot analysis (D).