Figure 2.

Replication of mouse RML prions in CAD5-PrP^−/−(MoPrP) cells. A, immunoblot of PK-digested cellular homogenates from either uninfected CAD5 cells (Ctrl) or RML prion–infected CAD5 cells (RML). B, infection of CAD5-PrP^−/− and CAD5-PrP^−/−(MoPrP) cells with RML prions. Immunoblots of either undigested (left blot) or PK-digested (right blot) lysates from cells at passage seven following exposure to homogenates from either uninfected CAD5 cells or RML prion-infected CAD5 cells. In both panels, PK-resistant PrP was visualized using the antibody HuM-P, and total (undigested) PrP was detected using the antibody HuM-D13. The undigested blot was also reprobed with an antibody to actin (20–33). Molecular mass measurements are indicated in kDa.