Figure 2.

Insulin-induced Pomc gene expression in middle age versus DIO conditions. Following overnight fasting, 3-month-old chow-fed, 3-month-old HFD-fed, and 15-month-old chow-fed mice were infused in the hypothalamic third ventricle with vehicle (Veh) or insulin (INS). At 90 min post-infusion, the hypothalamic samples were harvested for measurements of Pomc mRNA (A), Agrp mRNA (B), Npy mRNA (C), and Nt mRNA (D). *, p < 0.05; ***, p < 0.001, n = 8–10 per group (biological replicates). Error bars reflect mean ± S.E. AU, arbitrary unit. NS, not significant.