Figure 4.

Gel mobility shift assays of TraB_s and TraB_sΔγ with G-quadruplex DNA. A, G-quadruplex DNA substrate, formed with oligonucleotide I as described under “Experimental procedures,” was incubated with increasing concentrations of TraB_s or TraB_sΔγ proteins at 0, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 3, 4, and 5 μm. B, affinity curves obtained from the DNA-binding shift assays in the presence of G-quadruplex DNA (black squares), dsDNA with the clt sequence (black dots), and dsDNA with three overlapping TRS sequences (black triangles). Data represent the average of at least three different experiments. Error bars: S.D.