Figure 6.

3D reconstruction of TraB_s oligomer. A, TraB_s from peak 1 was negatively stained with uranyl acetate and analyzed by EM. Particles were selected, aligned, and classified by maximum likelihood methods. A 3D reconstruction was obtained by projection matching, with imposed C₄ symmetry in the final iterations. Scale bar represents 100 nm. B, fitting of TraB_s in the EM maps. An atomic model of TraB_s was generated by using atomic coordinates from FtsK of P. aeruginosa as template (2iuu.pdb and 2ve9.pdb coordinates for the motor domain and the γ-domain, respectively). The atomic coordinates were docked into the EM maps using SITUS package software.