Figure 5.

Deletion of Munc18-2 impedes platelet aggregation and interferes with thrombus formation in vitro. A and B, samples from Munc18-2 mutant mice were stimulated with collagen. Representative tracings of platelet aggregation (A) and maximum aggregation measured by light transmittance (B) on platelet-rich plasma. n = 6. C–F, whole blood was labeled fluorescently and perfused over collagen-coated plates at low (C and D) or high (E and F) shear stress. Thrombus buildup (C and E) was monitored by the change in fluorescence intensity over baseline (ΔMFI) and compared at 200 s (D and F). n = 5–8; AU, arbitrary units. The color legend in A applies to all panels. Circle or white line, mean; error bar, S.E.; box, 25th-75th percentile; whiskers, 5th–95th percentile. #, p < 0.05; †, p ≤ 0.01; *, p ≤ 0.001; comparisons are to WT controls (+/+) unless otherwise specified.