Table 1.

Technique comparison of clinical EV analysis.

Technique	Advantages	Limitations	References
Conventional Flow Cytometry	Widely available in clinical laboratories Clinical validated machines Possible to phenotype High throughput and automation Good reproducibility	Limited sensitivity Variability known to occur between different machines Requires expertise	[31,56]
High Sensitivity Flow Cytometry	Good resolution Possible to phenotype High throughput Good reproducibility	Expensive Requires expertise	[10,42,57,58]
Nanoparticle Tracking Analysis	High resolution Possible to phenotype Good reproducibility	Throughput Specificity – Designed for monodisperse populations	[10,18,59–62]
Dynamic Light Scattering	High resolution Good reproducibility	Throughput Specificity – Designed for monodisperse populations	[60,62–64]
Resistive Pulse Sensing	High resolution Good reproducibility	Throughput Specificity – Designed for monodisperse populations	[10,62,65]
Electron Microscopy	High resolution Possible to phenotype	Not widely available Expensive Throughput Poor Reproducibility	[11]