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. 2019 Mar 21;8(1):1587567. doi: 10.1080/20013078.2019.1587567

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Imaging flow cytometry facilitates single EV detection and alongside with cells in unprocessed samples. (a) IFCM analysis with dotplots and example event images gated on respective regions R1-R5 of complete suspension cultures derived from (a) THP-1:CD63eGFP cells. (b) Measured concentrations of gated populations in (a) and ratios expressed as percentages of total events gated within R1-R5. Measurements of unprocessed samples of suspension cultures from (c) THP-1:eGFP cells, and (d) unmanipulated THP-1 cells are shown for comparison. The data presented shows one out of three independently performed experiments with comparable outcome.