Table 1.

Acquisition parameters for Gigamix bead measurements with conventional flow cytometers.

Instrument	SSC laser	SSC threshold	SSC detector voltage (V)	eGFP filter (nm)	eGFP detector voltage (V)
Beckman Coulter FC500	488 nm; 20 mW	2000	1000	525/40	800
BD Accuri C6	488 nm; 20 mW	100	n/a*	533/30	n/a*
ACEA Novocyte 3000	488 nm; 20 mW	100	n/a*	530/30	n/a*
Beckman Coulter Gallios	488 nm; 22 mW	1000	1000	525/40	700
BD FACSAria IIIu	488 nm; 22 mW	200	450	530/30	800
Beckman Coulter Cytoflex	405 nm; 80 mW	1000**	*/Gain: 61	525/40	*/Gain: 2200

*Detector voltages are preset and couldn’t be changed.