Skip to main content
. 2019 Feb 26;41:636–648. doi: 10.1016/j.ebiom.2019.02.013

Fig. 5.

Fig. 5

Fig. 5

Silencing and overexpressing genes in the NFκB pathway and effect on sFlt-1 and sENG secretion.

Silencing NFκB in cytotrophoblast cells significantly reduced a) NFκB expression however this did not change b) sFlt-1 secretion or c) sFlt-1 e15a mRNA expression or d) sENG secretion. Overexpression of NFκB p65 resulted in an increase in e) Rel A expression however no change in f) sFlt-1 secretion, g) sFlt-1 e15a expression or h) sENG secretion. Furthermore overexpression of IKBKB in cytotrophoblast cells resulted in an increase in i) IKBKB expression however there was no change in j) sFlt-1 secretion, k) sFlt-1 e15a expression or l) sENG secretion. Data are means ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (t-test).