Figure 7.

RIG-I-Like signaling and apoptosis are increased in kidneys of Six2^Cre Dnmt1^f/f mice. (A) The GSEA enrichment plots of RNA sequencing data showing enrichment for RIG-I–like signaling in Six2^Cre Dnmt1^f/f mice. (B) Relative expression level of genes involved in RIG-I–like signaling. y-axis is normalized expression level determined by RNA sequencing. Asterisks represent significant differences calculated by DESeq2. (C) The GSEA enrichment plots of RNA sequencing data of P53 signaling in Six2^Cre Dnmt1^f/f mice. (D) Relative expression level of genes involved in p53 signaling. y-axis is normalized expression level determined by RNA sequencing. Asterisks represent significant differences calculated by DESeq2. (E) Left panel: immunofluorescence staining of P53 (red) and TUNEL assay (red) in P0 Dnmt1^f/f and Six2^Cre Dnmt1^f/f mice kidneys; right panel: quantification of P53- and TUNEL-positive signaling according to the staining. (F) Left panel: immunofluorescence staining of Ki67 (red) in P0 Dnmt1^f/f and Six2^Cre Dnmt1^f/f mice kidneys; right panel: quantification of Ki67-positive cells. (G) Schematic of our model which Dnmt1 deletion in progeny of Six2-positive cells affects kidney development. *P<0.05 and ***P<0.001.