Figure 3.

Tubular glucose-induced TGF inhibition is mediated via macula densa SGLT1. SGLT1 on macula densa cells was identified by immunofluorescence staining in human kidney biopsy specimens. (A) Positive staining of SGLT1 (red) was observed in macula densa cells marked with NOS1 antibody (green). (B and C) SGLT1 inhibitor KGA-2727 blocked the inhibitory effect of luminal glucose on the TGF response in vitro in isolated perfused JGA from mouse kidney; n=10–12; *P<0.01 versus baseline. Statistical difference was calculated by two-way ANOVA followed by multiple comparisons post hoc test.