Figure 4.

Tubular glucose stimulates NO generation in the macula densa. The macula densa NO generation was measured in isolated perfused JGA with DAF-2 DA. In the presence of glucose in tubular perfusate, NO generation in the macula densa was increased from 118.3±7.7 to 257±9.2 U/min. NO generation was not significantly altered by luminal L-glucose or mannitol. (A) Increased glucose concentration in the bath had no significant effect on NO generation in the macula densa; n=13–15; *P<0.01 versus baseline. (B) SGLT1 inhibitor KGA-2727 blocked the glucose-induced NO generation at the macula densa in isolated perfused JGA from mouse kidney; n=10–15; *P<0.01 versus baseline. Statistical difference was calculated by two-way ANOVA followed by multiple comparisons post hoc test.