Fig. 5.

Truncating mutations in polycystic kidney and hepatic disease 1 (PKHD1) or polycystic kidney disease 2 (PKD2) genes have no major effect on human embryonic kidney (HEK-293) cell proliferation. A: PKHD1 gene truncations had no overall effect on proliferation rate estimated by thiazolyl blue tetrazolium bromide (MTT) proliferation assay [vs. wild-type (WT): b = 0.09, P = 0.2499]. The 626* label designates the PKHD1 p.K626_M627del clone that was not included in these statistical analyses; however, when it was included, results were nearly identical. B: PKD2 gene mutations had no overall effect on MTT-based proliferation rates (vs. WT: b = 0.02, P = 0.5452).