Table 2.

Oligonucleotides used for PKHD1 and PKD2 targeting

Mutation (cDNA)	CRISPR gRNA Sequence
PKHD1
c.126delT	3′-GTAGCCTTGCAGGGGGAACGTGG-5′
c.711_714del	3′-CATGTGGAAGGCGACTACATCGG-5′
c.1854delA	5′-TGAAGGACACAATCATCTTCAGG-3′
c.3761_3762delCCinsG	5′-TGAAGGACACAATCATCTTCAGG-3′
c.9689delA	5′-GCATCGGGTATCTGGGGGGCTGG-3′
PKD2
c.958C>T	5′-ACTCGGAGTTGCCGTATTCGTGG-3′
c.2224C>T	3′-GAACTTCGACAAGATCTCAAAGG-5′
c.2407C>T	5′-CAGAGTCATCCAGGCTTCGAGGG-3′
c.2419C>T	5′-CAGAGTCATCCAGGCTTCGAGGG-3′

Protospacer adjacent motifs are underlined. PKHD1, polycystic kidney and hepatic disease 1 gene; PKD2, polycystic kidney disease 2 gene; gRNA, guide RNA.