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Effect of increasing media osmolarity for 30 min or 2 h (300 vs. 450 mosM using mannitol) on NFAT5 cytoplasmic and nuclear protein content. The cytoplasmic fraction Western blot and densitometry are shown in A and C, respectively; the nuclear fraction Western blot and densitometry and shown in B and D, respectively. n = 3 for each data point, except n = 2 for nuclear fraction 2 h. *P < 0.05 vs. 300 mosM. TBP, TATA-binding protein.
