Figure 4.

Haplotype reconstruction and phasing. (A) Inferred length-weighted mean molecule length plotted against N50 of called Phase blocks (both metrics reported by Long Ranger) and differentiated by sample ID and heterozygosity. Heterozygosity was calculated by dividing the total number of heterozygous positions called by Long Ranger by the total number of non-N bases in the reference genome (GRCh37). Two replicates of NA19240 and five replicates of NA12878 were used. Samples with higher heterozygosity produce longer phase blocks than samples with less diversity when controlling for input molecule length. (B) Phase block distributions across the genome for input length matched Chromium Genome samples NA12878 and NA19240. Phase blocks are shown as displayed in Loupe Genome Browser. Solid colors indicate phase blocks. Note the longer phase blocks in the more diverse NA19240 sample.